TAZ interacts with zonula occludens-1 and -2 proteins in a PDZ-1 dependent manner

Eline Remue; Kris Meerschaert; Tsutomu Oka; Ciska Boucherie; Joël Vandekerckhove; Marius Sudol; Jan Gettemans

doi:10.1016/j.febslet.2010.09.020

TAZ interacts with zonula occludens-1 and -2 proteins in a PDZ-1 dependent manner

FEBS Lett. 2010 Oct 8;584(19):4175-80. doi: 10.1016/j.febslet.2010.09.020. Epub 2010 Sep 18.

Authors

Eline Remue¹, Kris Meerschaert, Tsutomu Oka, Ciska Boucherie, Joël Vandekerckhove, Marius Sudol, Jan Gettemans

Affiliation

¹ Department of Medical Protein Research, VIB, Gent, Belgium.

PMID: 20850437
DOI: 10.1016/j.febslet.2010.09.020

Abstract

The transcriptional coactivator TAZ recognizes L/PPxY motifs in transcription factors like Runx1/2 through its WW domain. We show that the first PDZ domain of zona occludens-1 (ZO-1) and 2 (ZO-2) interacts with the carboxy-terminal PDZ binding motif of TAZ. Deletion of this motif abrogates binding. ZO-2 colocalizes with TAZ in the nucleus of MDCK cells and ZO-2 expression alters TAZ localization in human embryonic kidney cells. Luciferase assays demonstrate ZO-2 inhibition of TAZ-mediated transactivation. We propose that zonula occludens is a negative regulator of TAZ and suggest that selected tight junction proteins control nuclear translocation and activity of TAZ.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Active Transport, Cell Nucleus
Acyltransferases
Animals
Cell Line
Dogs
Humans
Intracellular Signaling Peptides and Proteins / chemistry
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / metabolism
Membrane Proteins / chemistry
Membrane Proteins / genetics
Membrane Proteins / metabolism*
PDZ Domains
Phosphoproteins / chemistry
Phosphoproteins / genetics
Phosphoproteins / metabolism*
Protein Interaction Domains and Motifs
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Sequence Deletion
Trans-Activators
Transcription Factors / chemistry
Transcription Factors / genetics
Transcription Factors / metabolism*
Transcriptional Activation
Transcriptional Coactivator with PDZ-Binding Motif Proteins
Zonula Occludens-1 Protein
Zonula Occludens-2 Protein

Substances

Intracellular Signaling Peptides and Proteins
Membrane Proteins
Phosphoproteins
Recombinant Fusion Proteins
TJP1 protein, human
TJP2 protein, human
Trans-Activators
Transcription Factors
Transcriptional Coactivator with PDZ-Binding Motif Proteins
WWTR1 protein, human
Zonula Occludens-1 Protein
Zonula Occludens-2 Protein
Acyltransferases
TAFAZZIN protein, human