Interferon-γ secretion by t(9;22) acute lymphoblastic leukemia-derived dendritic cells

Leuk Res. 2011 Feb;35(2):275-7. doi: 10.1016/j.leukres.2010.09.003. Epub 2010 Oct 12.

Abstract

Interferon (IFN)-γ plays an important role in immunity and anti-tumor activity. It is produced by lymphocytes, but was recently shown to be also produced by human myeloid dendritic cells (DCs). We have shown that human mature t(9;22) acute lymphoblastic leukemia-derived (ALL) DCs induced autologous cytotoxic T cell responses and therefore asked whether t(9;22) ALL-DC secreted IFN-γ. IFN-γ varied among three cell line-derived ALL-DCs; median production from seven patient ALL-DCs was 3450 pg/ml (range 1450-8675). IFN-γ production was dependent on maturation of ALL-DCs. This is the first demonstration of IFN-γ production by t(9;22) ALL-DCs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Separation
  • Cells, Cultured
  • Chromosomes, Human, Pair 22 / genetics*
  • Chromosomes, Human, Pair 9 / genetics*
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism*
  • Flow Cytometry
  • Humans
  • Interferon-gamma / metabolism*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / immunology*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism*

Substances

  • Interferon-gamma