Abstract
Different cytokines have been suggested to be involved in the pathogenesis of pulmonary tuberculosis (TB). The frequencies of Mycobacterium tuberculosis (MTB) specific CD4(+) and CD8(+) T cells, CD4(+)CD25(+) Forkhead Box Protein (FoxP)3(+) T cells, interleukin (IL)-6, interferon (IFN)-γ, Tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β and IL-10 were assessed in HIV-negative, pulmonary tuberculosis (TB) patients (n=30) and in healthy controls (n=23) in Gabon. Peripheral blood mononuclear cells (PBMC) were stimulated with purified protein derivative (PPD) and early secretory antigenic target-6 (ESAT-6). In patients, a pronounced pro-inflammatory cytokine response with highly significant increased levels of IL-6 and TNF-α accompanied by increased TGF-β was detectable. Differences in IFN-γ responses between patients and healthy individuals were less pronounced than expected. FoxP3 expression did not differ between groups. A distinct cytokine pattern is associated with active pulmonary TB in patients from Central Africa.
Copyright © 2010 Elsevier Inc. All rights reserved.
MeSH terms
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Adolescent
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Adult
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Aged
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Antigens, Bacterial / immunology
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Antigens, Bacterial / pharmacology
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Bacterial Proteins / immunology
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Bacterial Proteins / pharmacology
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Body Temperature
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / drug effects
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism
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CD8-Positive T-Lymphocytes / cytology
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CD8-Positive T-Lymphocytes / drug effects
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism
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Cell Count
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Cytokines / blood
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Cytokines / metabolism*
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Female
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Forkhead Transcription Factors / genetics
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Forkhead Transcription Factors / metabolism
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Gabon
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Gene Expression / drug effects
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Gene Expression / genetics
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Hemoglobins / metabolism
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Humans
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Interferon-gamma / blood
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Interferon-gamma / metabolism
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Interleukin-10 / blood
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Interleukin-10 / metabolism
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Interleukin-2 / metabolism
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Interleukin-6 / blood
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Interleukin-6 / metabolism
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Leukocytes, Mononuclear / drug effects
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / metabolism
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Male
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Middle Aged
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T-Lymphocyte Subsets / cytology
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T-Lymphocyte Subsets / drug effects
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism
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Transforming Growth Factor beta / blood
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Transforming Growth Factor beta / metabolism
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Tuberculin / immunology
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Tuberculin / pharmacology
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Tuberculosis, Pulmonary / blood
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Tuberculosis, Pulmonary / immunology
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Tuberculosis, Pulmonary / metabolism*
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Tumor Necrosis Factor-alpha / blood
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Tumor Necrosis Factor-alpha / metabolism
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Young Adult
Substances
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Antigens, Bacterial
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Bacterial Proteins
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Cytokines
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ESAT-6 protein, Mycobacterium tuberculosis
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FOXP3 protein, human
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Forkhead Transcription Factors
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Hemoglobins
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IL10 protein, human
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IL6 protein, human
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Interleukin-2
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Interleukin-6
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Transforming Growth Factor beta
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Tuberculin
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Interferon-gamma