Aberrant expression of CKLF-like MARVEL transmembrane member 5 (CMTM5) by promoter methylation in myeloid leukemia

Jihong Niu; Henan Li; Yao Zhang; Jinlan Li; Min Xie; Lingdi Li; Xiaoying Qin; Yazhen Qin; Xiaohuan Guo; Qian Jiang; Yanrong Liu; Shanshan Chen; Xiaojun Huang; Wenling Han; Guorui Ruan

doi:10.1016/j.leukres.2010.11.023

Aberrant expression of CKLF-like MARVEL transmembrane member 5 (CMTM5) by promoter methylation in myeloid leukemia

Leuk Res. 2011 Jun;35(6):771-6. doi: 10.1016/j.leukres.2010.11.023. Epub 2010 Dec 18.

Authors

Jihong Niu¹, Henan Li, Yao Zhang, Jinlan Li, Min Xie, Lingdi Li, Xiaoying Qin, Yazhen Qin, Xiaohuan Guo, Qian Jiang, Yanrong Liu, Shanshan Chen, Xiaojun Huang, Wenling Han, Guorui Ruan

Affiliation

¹ Peking University People's Hospital and Institute of Hematology, Beijing, China.

PMID: 21168207
DOI: 10.1016/j.leukres.2010.11.023

Abstract

CMTM5 has been shown to exhibit tumor suppressor activities, however, its role in leukemia is unclear. Herein we firstly reported the expression and function of CMTM5 in myeloid leukemia. CMTM5 was down-regulated, or undetectable, in leukemia cell lines and bone marrow cells from leukemia patients with promoter methylation. Ectopic expression of CMTM5-v1 strongly inhibited the proliferation of K562 and MEG-01 cells. In addition, significant negative correlations were observed between CMTM5 and three leukemia-specific fusion genes (AML1-ETO, PML-RARα and BCR/ABL1). CMTM5 expression was up-regulated in patients who had undergone treatment. Therefore, CMTM5 may be involved in the pathomechanism of myeloid leukemias.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antineoplastic Combined Chemotherapy Protocols / therapeutic use
Blotting, Western
Bone Marrow Cells / metabolism
Bone Marrow Cells / pathology
Cell Line, Tumor
Chemokines / genetics*
Chemokines / metabolism
Core Binding Factor Alpha 2 Subunit / genetics
DNA Methylation*
Down-Regulation
Female
Fusion Proteins, bcr-abl / genetics
Gene Expression Regulation, Leukemic*
HL-60 Cells
Humans
K562 Cells
Leukemia, Myeloid, Acute / drug therapy
Leukemia, Myeloid, Acute / genetics*
Leukemia, Myeloid, Acute / pathology
MARVEL Domain-Containing Proteins
Male
Oncogene Proteins, Fusion / genetics
Promoter Regions, Genetic / genetics*
RUNX1 Translocation Partner 1 Protein
Remission Induction
Reverse Transcriptase Polymerase Chain Reaction
Tumor Suppressor Proteins / genetics*
Tumor Suppressor Proteins / metabolism
Up-Regulation / drug effects
Young Adult

Substances

AML1-ETO fusion protein, human
CMTM5 protein, human
Chemokines
Core Binding Factor Alpha 2 Subunit
MARVEL Domain-Containing Proteins
Oncogene Proteins, Fusion
RUNX1 Translocation Partner 1 Protein
Tumor Suppressor Proteins
promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein
Fusion Proteins, bcr-abl