Variation in the ADH1B proximal promoter affects expression

Chem Biol Interact. 2011 May 30;191(1-3):38-41. doi: 10.1016/j.cbi.2010.12.007. Epub 2010 Dec 17.

Abstract

The primary pathway of metabolism of dietary alcohol is via its oxidation in liver by alcohol dehydrogenases (ADH). Differences in the ADH enzyme activity or levels of enzyme present could affect the risk for alcoholism. Regulatory variations have been shown to affect the promoter activity and thereby affect the risk for alcoholism. In this study the functional effects of the two SNPs (rs1159918 and rs1229982) in the proximal promoter region of ADH1B that were associated with alcoholism were explored. We examined the effects of five naturally occurring haplotypes on the promoter activity. We observed that a C to A change at rs1229982 increased promoter activity 1.4-fold.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alcohol Dehydrogenase / genetics*
  • Alleles
  • Gene Expression Regulation, Enzymologic / genetics*
  • Haplotypes
  • Hep G2 Cells
  • Humans
  • Polymorphism, Single Nucleotide / genetics*
  • Promoter Regions, Genetic / genetics*

Substances

  • ADH1B protein, human
  • Alcohol Dehydrogenase