MOHITO, a novel mouse cytokine-dependent T-cell line, enables studies of oncogenic signaling in the T-cell context

Maria Kleppe; Nicole Mentens; Thomas Tousseyn; Iwona Wlodarska; Jan Cools

doi:10.3324/haematol.2010.035931

MOHITO, a novel mouse cytokine-dependent T-cell line, enables studies of oncogenic signaling in the T-cell context

Haematologica. 2011 May;96(5):779-83. doi: 10.3324/haematol.2010.035931. Epub 2010 Dec 29.

Authors

Maria Kleppe¹, Nicole Mentens, Thomas Tousseyn, Iwona Wlodarska, Jan Cools

Affiliation

¹ Department of Molecular and Developmental Genetics, VIB, Leuven, Belgium.

Abstract

The mouse pro-B cell line Ba/F3 has gained major interest as a model system to investigate oncogenic tyrosine kinases and to determine the efficacy of kinase inhibitors. While Ba/F3 cells are suitable to study oncogenic kinases derived from various cell types, the signaling networks in Ba/F3 cells are B-cell specific. We have established a mouse CD4+CD8+ double positive T-cell line (named MOHITO, for MOuse Hematopoietic Interleukin-dependent cell line of T-cell Origin) that has many features of human T-cell acute lymphoblastic leukemia (Notch1 and Jak1 mutation, TCR rearrangement) and is dependent on interleukin-7. The MOHITO cell line can be transformed to cytokine independent proliferation by BCR-ABL1 or mutant JAK1. This mouse T-cell line is a novel model system to investigate protein signaling and inhibition in a T-cell specific context and is a valuable tool to study and verify oncogenic capacity of mutations in the kinome and phosphatome in T-cell malignancies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis / drug effects
Blotting, Western
CD4 Antigens / metabolism
CD8 Antigens / metabolism
Carcinogens / metabolism*
Cell Line
Cell Line, Tumor
Cell Proliferation / drug effects
Female
Fusion Proteins, bcr-abl / genetics
Fusion Proteins, bcr-abl / metabolism
HEK293 Cells
Humans
In Situ Hybridization, Fluorescence
Interleukin-7 / metabolism*
Interleukin-7 / pharmacology
Janus Kinase 1 / genetics
Janus Kinase 1 / metabolism
Karyotyping
Mice
Mice, Inbred BALB C
Mutation
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / genetics
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / metabolism
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / pathology
Receptor, Notch1 / genetics
Receptor, Notch1 / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction*
T-Lymphocytes / metabolism*

Substances

CD4 Antigens
CD8 Antigens
Carcinogens
Interleukin-7
Receptor, Notch1
Fusion Proteins, bcr-abl
Janus Kinase 1