Abstract
A male infertility-linked human PLCζ (phospholipase Cζ) mutation introduced into mouse PLCζ completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca2+ oscillations in mouse eggs. Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism.
© The Authors Journal compilation © 2011 Biochemical Society
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium / metabolism*
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Calcium Signaling
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Female
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Humans
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Hydrolysis
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Infertility, Male / genetics*
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Male
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Mice
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Oocytes / metabolism
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Phosphatidylinositol 4,5-Diphosphate / metabolism*
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Phosphoinositide Phospholipase C / genetics*
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Phosphoinositide Phospholipase C / metabolism*
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Point Mutation*
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Spermatozoa / enzymology*
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Spermatozoa / metabolism
Substances
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Phosphatidylinositol 4,5-Diphosphate
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PLCZ1 protein, human
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Phosphoinositide Phospholipase C
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Plcz1 protein, mouse
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Calcium