Rapid diagnosis of tuberculous meningitis by polymerase chain reaction (PCR)

K Kaneko; O Onodera; T Miyatake; S Tsuji

doi:10.1212/wnl.40.10.1617

Rapid diagnosis of tuberculous meningitis by polymerase chain reaction (PCR)

Neurology. 1990 Oct;40(10):1617-8. doi: 10.1212/wnl.40.10.1617.

Authors

K Kaneko¹, O Onodera, T Miyatake, S Tsuji

Affiliation

¹ Department of Neurology, Niigata University, Japan.

PMID: 2120615
DOI: 10.1212/wnl.40.10.1617

Abstract

Using a rapid and highly sensitive method for the diagnosis of tuberculous meningitis by polymerase chain reaction (PCR), we detected the Mycobacterium tuberculosis genome in CSF of 5 of 6 patients with clinically diagnosed tuberculous meningitis. The genome was not present in CSF from 10 patients with other types of meningitis and 10 normal controls.

MeSH terms

Adult
Cerebrospinal Fluid / microbiology
DNA, Bacterial / analysis
Female
Humans
Male
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification
Polymerase Chain Reaction / methods*
Time Factors
Tuberculosis, Meningeal / diagnosis*
Tuberculosis, Meningeal / microbiology

Substances

DNA, Bacterial