PANP is a novel O-glycosylated PILRα ligand expressed in neural tissues

Biochem Biophys Res Commun. 2011 Feb 18;405(3):428-33. doi: 10.1016/j.bbrc.2011.01.047. Epub 2011 Jan 15.

Abstract

PILRα is an immune inhibitory receptor possessing an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain enabling it to deliver inhibitory signals. Binding of PILRα to its ligand CD99 is involved in immune regulation; however, whether there are other PILRα ligands in addition to CD99 is not known. Here, we report that a novel molecule, PILR-associating neural protein (PANP), acts as an additional ligand for PILRα. Transcription of PANP was mainly observed in neural tissues. PILRα-Ig fusion protein bound cells transfected with PANP and the transfectants stimulated PILRα reporter cells. Specific O-glycan structures on PANP were found to be required for PILR recognition of this ligand. These results suggest that PANP is involved in immune regulation as a ligand of the PILRα.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Humans
  • Ligands
  • Melanoma, Experimental
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Rats
  • Rats, Wistar
  • Receptors, Immunologic / metabolism*

Substances

  • Ligands
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • PIANP protein, human
  • PILRA protein, human
  • PILRalpha protein, mouse
  • Pianp protein, mouse
  • Receptors, Immunologic