Abstract
Latent infection of human T-cell leukemia virus type 1 (HTLV-1) is considered to be preferentially associated with CCR4(+) CD4(+) T cells. Here we report that c-Maf, one of the critical transcription factors for Th2 differentiation, suppresses the transcriptional activity of HTLV-1 Tax by competing for CREB-binding protein. Notably, c-maf expression is selectively induced in a fraction of CCR4(+) CD4(+) T cells upon activation. Furthermore, c-Maf significantly decreases Tax-induced HTLV-1 envelope gp46 gene expression from an infectious HTLV-1 molecular clone and tax expression in a cell-free HTLV-1 infection system. Collectively, c-Maf may play a role in latent infection of HTLV-1 in CCR4(+) CD4(+) T cells by negatively regulating Tax activity.
© 2011 Japanese Cancer Association.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Western
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / virology
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CREB-Binding Protein / genetics
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CREB-Binding Protein / metabolism*
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Cell Transformation, Viral
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Gene Products, env / genetics
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Gene Products, env / metabolism
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Gene Products, tax / antagonists & inhibitors
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Gene Products, tax / genetics
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Gene Products, tax / metabolism*
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HTLV-I Infections / genetics
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HTLV-I Infections / metabolism*
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HTLV-I Infections / virology
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Human T-lymphotropic virus 1 / genetics
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Human T-lymphotropic virus 1 / metabolism*
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Humans
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Jurkat Cells
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Leukemia-Lymphoma, Adult T-Cell / genetics
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Leukemia-Lymphoma, Adult T-Cell / metabolism
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Leukemia-Lymphoma, Adult T-Cell / virology
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Luciferases / metabolism
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Promoter Regions, Genetic
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Proto-Oncogene Proteins c-maf / antagonists & inhibitors
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Proto-Oncogene Proteins c-maf / genetics
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Proto-Oncogene Proteins c-maf / metabolism*
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RNA, Messenger / genetics
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RNA, Small Interfering / genetics
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Receptors, CCR4 / genetics
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Receptors, CCR4 / metabolism
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Retroviridae Proteins, Oncogenic / genetics
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Retroviridae Proteins, Oncogenic / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Th2 Cells
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Transcriptional Activation
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Virion
Substances
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CCR4 protein, human
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Gene Products, env
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Gene Products, tax
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MAF protein, human
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Proto-Oncogene Proteins c-maf
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RNA, Messenger
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RNA, Small Interfering
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Receptors, CCR4
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Retroviridae Proteins, Oncogenic
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gp46 protein, Human T-cell leukemia virus type I
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Luciferases
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CREB-Binding Protein
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CREBBP protein, human