Black tea polyphenol inhibits CXCL10 production in oncostatin M-stimulated human gingival fibroblasts

Yoshitaka Hosokawa; Ikuko Hosokawa; Satoru Shindo; Kazumi Ozaki; Tadashi Nakanishi; Hideaki Nakae; Takashi Matsuo

doi:10.1016/j.intimp.2011.01.009

Black tea polyphenol inhibits CXCL10 production in oncostatin M-stimulated human gingival fibroblasts

Int Immunopharmacol. 2011 Jun;11(6):670-4. doi: 10.1016/j.intimp.2011.01.009. Epub 2011 Jan 19.

Authors

Yoshitaka Hosokawa¹, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki, Tadashi Nakanishi, Hideaki Nakae, Takashi Matsuo

Affiliation

¹ Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Japan. hosokawa@dent.tokushima-u.ac.jp

PMID: 21255696
DOI: 10.1016/j.intimp.2011.01.009

Abstract

CXC chemokine ligand 10 (CXCL10) plays an important role in the infiltration of Th1 cells and thus in the exacerbation of periodontal disease. Theaflavin-3,3'-digallate (TFDG), polyphenol in black tea, has some beneficial effects but the effect of TFDG on CXCL10 production from human gingival fibroblasts (HGFs) is uncertain. In this study, we investigated the mechanisms by which TFDG may inhibit oncostatin M (OSM)-induced CXCL10 production in human gingival fibroblasts. TFDG prevented OSM-mediated CXCL10 production by HGFs in a dose dependent manner. TFDG significantly inhibited OSM-induced phosphorylation of c-Jun N terminal kinase (JNK), protein kinase B (Akt) (Ser473) that are related to CXCL10 production from OSM-stimulated HGFs. In addition, TFDG suppressed OSM receptor (OSMR) β expression on HGFs. These data provide a novel mechanism where the black tea flavonoid, theaflavin, could provide direct benefits in periodontal disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biflavonoids / pharmacology*
Catechin / pharmacology*
Cells, Cultured
Chemokine CXCL10 / genetics
Chemokine CXCL10 / metabolism
Fibroblasts / drug effects
Fibroblasts / metabolism*
Fibroblasts / pathology
Flavonoids / pharmacology*
Gallic Acid / analogs & derivatives*
Gallic Acid / pharmacology
Gingiva / pathology*
Humans
MAP Kinase Kinase 4 / metabolism
Oncostatin M / immunology
Oncostatin M / metabolism
Oncostatin M Receptor beta Subunit / genetics
Oncostatin M Receptor beta Subunit / metabolism
Periodontal Diseases / drug therapy*
Periodontal Diseases / immunology
Phenols / pharmacology*
Phosphorylation / drug effects
Polyphenols
Proto-Oncogene Proteins c-akt / metabolism
STAT3 Transcription Factor / metabolism
Tea

Substances

Biflavonoids
Chemokine CXCL10
Flavonoids
Oncostatin M Receptor beta Subunit
Phenols
Polyphenols
STAT3 Transcription Factor
Tea
Oncostatin M
theaflavin digallate
Gallic Acid
Catechin
Proto-Oncogene Proteins c-akt
MAP Kinase Kinase 4