Abstract
To explore the mechanisms of chemotherapy resistance, we previously established a multi-drug resistant cell line, Tca8113/Pingyangmycin (Tca8113/PYM) and identified differential expression in known genes and ESTs using microarray analysis. From among those ESTs we have now identified a novel gene producing an mRNA of 1834 nucleotides translated into a protein having 235 amino acids. This gene was denominated as tongue cancer resistance-associated protein 1 gene (TCRP1, accession number: EF363480). We further determined its functional characteristics. The results demonstrate that TCRP1 mediates a specific resistance to cisplatin in Tca8113 cells by reducing the cisplatin-induced apoptosis. This suggests that TCRP1 might be a novel molecular target to develop agents to reverse cisplatin-induced chemoresistance.
Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology
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Apoptosis / drug effects
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Blotting, Western
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cell Survival / drug effects
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Cisplatin / pharmacology*
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Cloning, Molecular
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Comet Assay
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Cytoplasm / metabolism
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DNA Damage
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DNA, Neoplasm / genetics
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Drug Resistance, Multiple / genetics*
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Drug Resistance, Neoplasm / genetics*
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Flow Cytometry
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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Microscopy, Fluorescence
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Proteins / genetics*
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Proteins / metabolism
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RNA Interference
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Reverse Transcriptase Polymerase Chain Reaction
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Tongue Neoplasms / genetics
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Tongue Neoplasms / metabolism
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Tongue Neoplasms / pathology
Substances
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Antineoplastic Agents
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DNA, Neoplasm
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Proteins
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tongue cancer resistance-associated protein 1, human
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Cisplatin