Assignment of the human gene for CREB1 to chromosome 2q32.3-q34

Genomics. 1990 Jul;7(3):416-21. doi: 10.1016/0888-7543(90)90176-u.

Abstract

The nuclear phosphoprotein cAMP-response-element-binding-protein 1 (CREB1) is involved in the transcriptional regulation of cAMP-inducible genes. We have determined the chromosomal location of the human CREB1 gene by using a cDNA probe. Southern blot analysis of genomic DNA from a panel of mouse-human somatic cell hybrids indicated that the CREB1 gene resides on human chromosome 2. Analysis of a somatic cell hybrid containing a chromosome 2 translocation narrowed assignment of the CREB1 locus to the terminal third of chromosome 2. Finally, in situ hybridization allowed localization of the gene to the 2q32.3-q34 region. This knowledge may prove valuable in molecular analysis of translocations occurring in this region and in exploring the possibility of a role for the CREB1 locus in malignancy.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Southern
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 2*
  • Cyclic AMP Response Element-Binding Protein
  • DNA-Binding Proteins / genetics*
  • Genes
  • Humans
  • Hybrid Cells
  • Mice
  • Translocation, Genetic

Substances

  • Cyclic AMP Response Element-Binding Protein
  • DNA-Binding Proteins