TGF-B1 pathway as biological marker of bladder carcinoma schistosomal and non-schistosomal

Olfat Shaker; Olfat Hammam; Mohamed Wishahi; Mamdouh Roshdi

doi:10.1016/j.urolonc.2011.02.003

TGF-B1 pathway as biological marker of bladder carcinoma schistosomal and non-schistosomal

Urol Oncol. 2013 Apr;31(3):372-8. doi: 10.1016/j.urolonc.2011.02.003. Epub 2011 Mar 23.

Authors

Olfat Shaker¹, Olfat Hammam, Mohamed Wishahi, Mamdouh Roshdi

Affiliation

¹ Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt. olfatshaker@yahoo.com

PMID: 21429769
DOI: 10.1016/j.urolonc.2011.02.003

Abstract

Objectives: Study TGF-β1 pathway in bladder carcinoma.

Design and methods: Eighty-one patients were enrolled: 16 chronic cystitis and 60 malignant bladder lesions; 15 schistosomal squamous cell carcinoma (SQCC), 45 transitional cell carcinoma (TCC). Five healthy individuals served as controls. mTGF-β1, protein, and its receptor expression in urine and bladder tissue were measured using in situ hybridization and immunohistochemical techniques, respectively.

Results: Overexpression of TGF-mRNA in invasive TCC group was compared with superficial TCC, high grade TCC was compared with low grade, and SQCC was compared with TCC. TGF-β1 protein and its receptor I (TGF-βR1) were overexpressed in urine samples in malignant group compared with chronic cystitis and in SQCC group compared with TCC group. TGF-ß1 protein and its receptor were significantly increased in schistosomal malignant group compared with non-schistosomal group.

Conclusion: Expression of TGF-ß1 and TGF-ßR1 could be used as biological markers of bladder carcinoma.

MeSH terms

Adult
Aged
Aged, 80 and over
Biomarkers, Tumor / genetics*
Biomarkers, Tumor / metabolism
Biomarkers, Tumor / urine
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism
Carcinoma, Squamous Cell / parasitology
Carcinoma, Transitional Cell / genetics
Carcinoma, Transitional Cell / metabolism
Carcinoma, Transitional Cell / parasitology
Female
Humans
Immunohistochemistry
In Situ Hybridization
Male
Middle Aged
Protein Serine-Threonine Kinases / metabolism
Protein Serine-Threonine Kinases / urine
Receptor, Transforming Growth Factor-beta Type I
Receptors, Transforming Growth Factor beta / metabolism
Schistosomiasis / genetics*
Schistosomiasis / metabolism
Schistosomiasis / parasitology
Signal Transduction
Transforming Growth Factor beta1 / genetics*
Transforming Growth Factor beta1 / metabolism
Transforming Growth Factor beta1 / urine
Urinary Bladder Neoplasms / genetics*
Urinary Bladder Neoplasms / metabolism
Urinary Bladder Neoplasms / parasitology

Substances

Biomarkers, Tumor
Receptors, Transforming Growth Factor beta
Transforming Growth Factor beta1
Protein Serine-Threonine Kinases
Receptor, Transforming Growth Factor-beta Type I