Abstract
Development of the segmented central nerve cords of vertebrates and invertebrates requires connecting successive neuromeres. Here, we show both how a pathway is constructed to guide pioneer axons between segments of the Drosophila CNS, and how motility of the pioneers along that pathway is promoted. First, canonical Notch signaling in specialized glial cells causes nearby differentiating neurons to extrude a mesh of fine projections, and shapes that mesh into a continuous carpet that bridges from segment to segment, hugging the glial surface. This is the direct substratum that pioneer axons follow as they grow. Simultaneously, Notch uses an alternate, non-canonical signaling pathway in the pioneer growth cones themselves, promoting their motility by suppressing Abl signaling to stimulate filopodial growth while presumably reducing substratum adhesion. This propels the axons as they establish the connection between successive segments.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, N.I.H., Intramural
MeSH terms
-
Animals
-
Animals, Genetically Modified
-
Axons / metabolism
-
Axons / physiology*
-
Cell Communication / genetics*
-
Cell Communication / physiology
-
Cell Differentiation / genetics
-
Cell Differentiation / physiology
-
Cell Growth Processes / genetics
-
Cell Growth Processes / physiology
-
Central Nervous System / cytology
-
Central Nervous System / embryology*
-
Central Nervous System / metabolism
-
Drosophila Proteins / genetics
-
Drosophila Proteins / metabolism
-
Drosophila Proteins / physiology*
-
Drosophila melanogaster / embryology*
-
Drosophila melanogaster / genetics
-
Drosophila melanogaster / metabolism
-
Drosophila melanogaster / physiology
-
Embryo, Nonmammalian
-
Growth Cones / metabolism
-
Growth Cones / physiology
-
Models, Biological
-
Nerve Growth Factors / metabolism
-
Netrin Receptors
-
Netrin-1
-
Neuroglia / metabolism
-
Neuroglia / physiology
-
Pseudopodia / genetics
-
Pseudopodia / metabolism
-
Pseudopodia / physiology
-
Receptors, Cell Surface / metabolism
-
Receptors, Notch / genetics
-
Receptors, Notch / metabolism
-
Receptors, Notch / physiology*
-
Synapses / genetics
-
Synapses / metabolism
-
Synapses / physiology
-
Tumor Suppressor Proteins / metabolism
Substances
-
Drosophila Proteins
-
N protein, Drosophila
-
Nerve Growth Factors
-
Netrin Receptors
-
Receptors, Cell Surface
-
Receptors, Notch
-
Tumor Suppressor Proteins
-
Netrin-1