Cultured Kaposi's sarcoma-derived cells express functional PDGF A-type and B-type receptors

Exp Cell Res. 1990 Mar;187(1):98-103. doi: 10.1016/0014-4827(90)90122-q.

Abstract

We have investigated the role of platelet-derived growth factor (PDGF) for the growth of cultured Kaposi's sarcoma (KS)-derived cells. They were found to bind 125I-PDGF-AA and 125I-PDGF-BB in a manner which is consistent with the presence of both PDGF A-type and PDGF B-type receptors. Furthermore, KS cells responded mitogenically to both PDGF homodimers. This shows that KS cells have functional A- and B-type PDGF receptors, suggesting an important role of PDGF for the growth of KS cells in vitro. In addition we found that KS cells produce PDGF-A chain mRNA in response to PDGF stimulation. This suggests that a PDGF-induced autocrine stimulation involving PDGF-AA which could amplify the response to PDGF occurs in these cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding, Competitive
  • Blotting, Northern
  • Cell Division / drug effects
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Gene Expression
  • Humans
  • Kinetics
  • Platelet-Derived Growth Factor / metabolism*
  • Platelet-Derived Growth Factor / pharmacology
  • RNA, Messenger / genetics
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Receptors, Platelet-Derived Growth Factor
  • Sarcoma, Kaposi / metabolism*
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism*

Substances

  • Platelet-Derived Growth Factor
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Platelet-Derived Growth Factor