Abstract
Prostate cancer (PCa) is one of the most common types of cancer in men in the United States and is the second leading cause of cancer related death in men. Clinically, secreted prostate specific antigen (PSA) has gained recognition because of its proteolytic activity being directly linked to PCa cell proliferation leading to disease initiation and progression. Using phage display technology, we identified four distinct cyclical peptides. These peptides apart from differences in their amino acid sequence, elicited minimal cross reactive antibody responses against each other. One of the four peptides analyzed produced an antibody response that recognizes the PSA protein. We demonstrate that the synthetic PSA peptide mimics identified in our study are immunologically active and produce neutralizing activity and this has relevance and utility for prostate cancer disease progression.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenocarcinoma / genetics
-
Adenocarcinoma / immunology*
-
Adenocarcinoma / metabolism
-
Amino Acid Sequence
-
Animals
-
Antibody Formation
-
Antibody Specificity
-
Antigens, Neoplasm / genetics
-
Antigens, Neoplasm / immunology*
-
Antigens, Neoplasm / metabolism
-
Blotting, Western
-
Cancer Vaccines / chemistry
-
Cancer Vaccines / genetics
-
Cancer Vaccines / immunology
-
Cell Line, Tumor
-
Enzyme-Linked Immunosorbent Assay
-
Humans
-
Male
-
Mice
-
Mice, Inbred BALB C
-
Molecular Mimicry
-
Molecular Sequence Data
-
Peptide Library
-
Peptides / chemistry*
-
Peptides / genetics
-
Peptides / immunology*
-
Peptides / metabolism
-
Prostate-Specific Antigen / genetics
-
Prostate-Specific Antigen / immunology*
-
Prostate-Specific Antigen / metabolism
-
Prostatic Neoplasms / genetics
-
Prostatic Neoplasms / immunology*
-
Prostatic Neoplasms / metabolism
Substances
-
Antigens, Neoplasm
-
Cancer Vaccines
-
Peptide Library
-
Peptides
-
Prostate-Specific Antigen