Differential utilization of calcitonin gene regulatory DNA sequences in cultured lines of medullary thyroid carcinoma and small-cell lung carcinoma

Mol Cell Biol. 1990 Apr;10(4):1773-8. doi: 10.1128/mcb.10.4.1773-1778.1990.

Abstract

Regulation of expression of the human calcitonin gene was found to differ between two tumor lines of different tissue origin, medullary thyroid carcinoma (TT line) and small-cell lung carcinoma (DMS53 line). Distal 5' DNA elements between -750 and -2000 exhibited a stronger basal activity in DMS53 than in TT cells, whereas proximal DNA sequences between -132 and -252 mediated a dramatic cyclic AMP response in TT but not DMS53 cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Calcitonin / genetics*
  • Carcinoma, Small Cell / genetics*
  • Cell Line
  • DNA, Neoplasm / genetics*
  • Gene Expression Regulation, Neoplastic*
  • Genes*
  • Humans
  • Lung Neoplasms / genetics*
  • Plasmids
  • Regulatory Sequences, Nucleic Acid*
  • Thyroid Neoplasms / genetics*
  • Transcription, Genetic
  • Tumor Cells, Cultured / metabolism

Substances

  • DNA, Neoplasm
  • Calcitonin