Clinical significance of aberrant DNA methylation in childhood acute lymphoblastic leukemia

Leuk Res. 2011 Oct;35(10):1345-9. doi: 10.1016/j.leukres.2011.04.015. Epub 2011 May 17.

Abstract

Methylation profile was analyzed in ninety-five patients with childhood acute lymphoblastic leukemia (ALL). Methylation of both MGMT and p16 genes were associated with higher age (p=0.01 and p=0.03, respectively). Methylation of both p15 and SHP1 genes occurred more frequently in T-ALL than in precursor B-ALL (p=0.02 and p=0.01, respectively). In contrast, methylation of the DAPK gene was more frequent in precursor B-ALL (p=0.01). Patients with methylation of multiple genes more likely had T cell phenotype, and are classified as medium/high risk (p=0.004 and p=0.03, respectively). These results suggest that methylation status is associated with clinicopathological features in childhood ALL.

Publication types

  • Multicenter Study

MeSH terms

  • Adolescent
  • Apoptosis Regulatory Proteins / genetics
  • Apoptosis Regulatory Proteins / metabolism
  • Biomarkers / blood*
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Case-Control Studies
  • Child
  • Cyclin-Dependent Kinase Inhibitor p15 / genetics
  • Cyclin-Dependent Kinase Inhibitor p15 / metabolism
  • DNA Methylation / genetics*
  • DNA Modification Methylases / genetics
  • DNA Modification Methylases / metabolism
  • DNA Repair Enzymes / genetics
  • DNA Repair Enzymes / metabolism
  • DNA, Neoplasm / blood
  • DNA, Neoplasm / metabolism*
  • Death-Associated Protein Kinases
  • Female
  • Genes, p16
  • Humans
  • Male
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / blood
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / diagnosis
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / genetics
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / pathology
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma* / blood
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma* / diagnosis
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma* / genetics
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma* / pathology
  • Promoter Regions, Genetic
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6 / genetics
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6 / metabolism
  • Retrospective Studies
  • Risk Factors
  • Tumor Suppressor Proteins / genetics
  • Tumor Suppressor Proteins / metabolism

Substances

  • Apoptosis Regulatory Proteins
  • Biomarkers
  • Cyclin-Dependent Kinase Inhibitor p15
  • DNA, Neoplasm
  • Tumor Suppressor Proteins
  • DNA Modification Methylases
  • MGMT protein, human
  • Death-Associated Protein Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • PTPN6 protein, human
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • DNA Repair Enzymes