Abstract
Receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) were identified on 9 of 35 (26%) human nonhematopoietic tumor cell lines including non-small cell lung cancer, stomach cancer, colon cancer, and osteosarcoma cells. GM-CSF receptors distributed on these human tumor cells were low affinity types with an equilibrium dissociation constant of 1.5-10.0 nM. Cross-linking studies revealed that the molecular weights of the low affinity GM-CSF receptors were 65-85 kilodaltons. The high affinity receptors identified on hematopoietic cells were not detected on human nonhematopoietic tumor cells which we studied, and we could detect no effects of GM-CSF on cell growth of these tumor cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinoma, Non-Small-Cell Lung / metabolism
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Carcinoma, Non-Small-Cell Lung / pathology
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Carcinoma, Non-Small-Cell Lung / ultrastructure*
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Cell Transformation, Neoplastic / metabolism
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Cell Transformation, Neoplastic / pathology
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology
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Colonic Neoplasms / ultrastructure*
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Gene Expression / physiology
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Humans
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology
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Lung Neoplasms / ultrastructure*
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Osteosarcoma / metabolism
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Osteosarcoma / pathology
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Osteosarcoma / ultrastructure*
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism*
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Receptors, Cell Surface / physiology
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Receptors, Colony-Stimulating Factor
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Stomach Neoplasms / metabolism
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Stomach Neoplasms / pathology
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Stomach Neoplasms / ultrastructure*
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Tumor Cells, Cultured / metabolism
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Tumor Cells, Cultured / pathology
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Tumor Cells, Cultured / ultrastructure
Substances
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Receptors, Cell Surface
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Receptors, Colony-Stimulating Factor