Abstract
One gene activator protein may interfere with the effects of another in eukaryotic cells. We report here that a hybrid yeast-herpes gene activator protein inhibits transcriptional activation by a thymidine-rich DNA element in yeast. This example of activator interference can be faithfully reproduced in vitro. Interference is reversed by a partially purified yeast component, but not by RNA polymerase II or various polymerase II transcription factors. We conclude that the partially purified yeast component is a novel factor, and we suggest this factor mediates the transcriptional activation process.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Nucleus / metabolism
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DNA, Fungal / genetics
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DNA-Binding Proteins
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Gene Expression Regulation, Fungal
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Kinetics
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Plasmids
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RNA Polymerase II / metabolism*
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Simplexvirus / genetics
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transcription Factors / metabolism*
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Transcription, Genetic*
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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GAL4 protein, S cerevisiae
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Phosphoproteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors
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RNA Polymerase II
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beta-Galactosidase