Abstract
The present studies investigated the expression of the platelet-derived growth factor (PDGF) and PDGF-receptor genes in human meningiomas. Northern blot analysis demonstrated that all meningiomas examined expressed both the c-sis/PDGF-2 proto-oncogene and the PDGF-receptor gene. In situ hybridization localized the c-sis mRNA and the PDGF-receptor mRNA in the tumor cells of the meningioma tissues. Control pachymeninges derived from adult individuals, without meningiomas, expressed only PDGF-receptor mRNA but not the c-sis mRNA. Immunocytochemistry studies detected both the c-sis and the PDGF-receptor protein products in meningioma tissues but only the PDGF-receptor protein products in control pachymeninges. These findings indicate the presence of an autocrine mechanism in human meningiomas based on the co-expression of the c-sis/PDGF-2 proto-oncogene and PDGF-receptor gene and their protein products. This co-expression of a potent mitogen and its receptor may contribute to the growth and maintenance of human meningiomas.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Blotting, Northern
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Gene Expression Regulation, Neoplastic / genetics*
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Gene Expression Regulation, Neoplastic / physiology
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Humans
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Immunohistochemistry
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Meningeal Neoplasms / genetics*
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Meningeal Neoplasms / metabolism
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Meningioma / genetics*
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Meningioma / metabolism
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Neoplasm Recurrence, Local / genetics*
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Neoplasm Recurrence, Local / metabolism
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Nucleic Acid Hybridization
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Oncogene Proteins / genetics*
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Oncogene Proteins / metabolism
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Platelet-Derived Growth Factor / genetics*
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Platelet-Derived Growth Factor / metabolism
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Proto-Oncogene Mas
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Proto-Oncogenes / genetics
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RNA, Messenger / genetics
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RNA, Neoplasm / genetics
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
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Receptors, Platelet-Derived Growth Factor
Substances
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MAS1 protein, human
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Oncogene Proteins
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Platelet-Derived Growth Factor
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Proto-Oncogene Mas
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RNA, Messenger
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RNA, Neoplasm
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Receptors, Cell Surface
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Receptors, Platelet-Derived Growth Factor