Abstract
Normal hepatocytes express very few class I major histocompatibility complex (MHC I) molecules, but MHC I expression is elevated in hepatitis B virus (HBV) infection. We report here that hepatoblastoma cells with replicating HBV genomes express three- to fourfold-higher levels of MHC I protein and mRNA than do parent cells without HBV DNA. Transient transfection assays demonstrated that the HBV X protein trans activated transcription from an MHC I promoter and allowed identification of cis elements important for trans activation.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
2',5'-Oligoadenylate Synthetase / genetics
-
Base Sequence
-
Carcinoma, Hepatocellular
-
Cell Line
-
Flow Cytometry
-
Gene Expression Regulation*
-
Genes, MHC Class I*
-
Hepatitis B / immunology*
-
Hepatitis B virus / genetics*
-
Hepatitis B virus / immunology
-
Histocompatibility Antigens Class I / analysis
-
Histocompatibility Antigens Class I / genetics
-
Humans
-
Liver Neoplasms
-
Molecular Sequence Data
-
Transcriptional Activation
Substances
-
Histocompatibility Antigens Class I
-
2',5'-Oligoadenylate Synthetase