Abstract
A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Carcinoma, Hepatocellular / chemically induced
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Carcinoma, Hepatocellular / genetics
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Carcinoma, Hepatocellular / metabolism*
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Cell Line, Tumor
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Diethylnitrosamine
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Female
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Gene Expression Profiling
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HEK293 Cells
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Humans
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Immunohistochemistry
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Liver / metabolism
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Liver / pathology
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Liver Neoplasms / chemically induced
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Liver Neoplasms / genetics
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Liver Neoplasms / metabolism*
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Luciferases, Firefly / genetics
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Luciferases, Firefly / metabolism
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Luminescent Measurements / methods*
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Molecular Imaging / methods*
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Promoter Regions, Genetic / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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alpha-Fetoproteins / genetics
Substances
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alpha-Fetoproteins
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Diethylnitrosamine
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Luciferases, Firefly