B7-H3 over expression in prostate cancer promotes tumor cell progression

J Urol. 2011 Sep;186(3):1093-9. doi: 10.1016/j.juro.2011.04.103. Epub 2011 Jul 23.

Abstract

Purpose: We compared B7-H3 expression in benign prostatic hyperplasia and prostate cancer tissue specimens, and determined the effects of low B7-H3 expression on the PC-3 human prostate cancer cell line using RNA interference.

Materials and methods: B7-H3 expression in prostate specimens was determined by enzyme-linked immunosorbent assay. A PC-3 cell line with low B7-H3 expression was established by RNA interference to investigate the effect of B7-H3 on cell proliferation, adhesion, migration and invasion in vitro.

Results: B7-H3 in tissue samples was significantly higher in the prostate cancer group than in the benign prostatic hyperplasia group (mean±SEM 174.73±56.80 vs 82.69±46.19 ng/gm, p<0.001). B7-H3 expression down-regulated by small interfering RNA decreased cell adhesion to PC-3 fibronectin more than 30%, and migration and Matrigel™ invasion up to 50%. No apparent impact was observed on cell proliferation.

Conclusions: B7-H3 is aberrantly expressed in prostate cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating PC-3 cell progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Antigens, CD / biosynthesis*
  • Antigens, CD / genetics*
  • B7 Antigens
  • Disease Progression
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • Middle Aged
  • Prostatic Hyperplasia / genetics
  • Prostatic Hyperplasia / pathology
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology*
  • Receptors, Immunologic / biosynthesis*
  • Receptors, Immunologic / genetics*

Substances

  • Antigens, CD
  • B7 Antigens
  • CD276 protein, human
  • Receptors, Immunologic