Structural studies of the O-antigenic polysaccharide from Escherichia coli O177

Carbohydr Res. 2011 Oct 18;346(14):2300-3. doi: 10.1016/j.carres.2011.06.022. Epub 2011 Jun 25.

Abstract

The structure of the O-antigen polysaccharide (PS) from Escherichia coli O177 has been determined. Component analysis together with (1)H and (13)C NMR spectroscopy experiments was used to determine the structure. Inter-residue correlations were determined by (1)H,(13)C-heteronuclear multiple-bond correlation and (1)H,(1)H-NOESY experiments. PS is composed of tetrasaccharide repeating units with the following structure: →2)-α-L-Rhap-(1→3)-α-L-FucpNAc-(1→3)-α-L-FucpNAc-(1→3)-β-D-GlcpNAc-(1→. An α-L-Rhap residue is suggested to be present at the terminal part of the polysaccharide, which on average is composed of ∼20 repeating units, since the (1)H and (13)C chemical shifts of an α-linked rhamnopyranosyl group could be assigned by a combination of 2D NMR spectra. Consequently, the biological repeating unit has a 3-substituted N-acetyl-D-glucosamine residue at its reducing end. The repeating unit of the E. coli O177 O-antigen shares the →3)-α-L-FucpNAc-(1→3)-β-D-GlcpNAc-(1→ structural element with the O-antigen from E. coli O15 and this identity may then explain the reported cross-reactivity between the strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Escherichia coli / chemistry*
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • O Antigens / chemistry*

Substances

  • O Antigens