Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2

Proc Natl Acad Sci U S A. 1990 Jul;87(13):5139-43. doi: 10.1073/pnas.87.13.5139.

Abstract

Entry into mitosis during the somatic cell cycle is regulated in response to signals that monitor the completion of DNA replication, the integrity of the nuclear genome, and, possibly, the increase in cellular mass during the cell cycle. It has been postulated that the operation of this cell cycle control involves the gradual accumulation of rate-limiting mitotic inducers, which trigger nuclear division when their cellular concentration reaches a critical level. We have cloned a human gene, which we call CDC25, whose product may function as a mitotic inducer. This human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25+ mitotic inducer. The human CDC25 gene rescues the defect of a fission yeast temperature-sensitive (ts) cdc25ts mutant that is unable to initiate mitosis. In HeLa cells CDC25 mRNA levels are very low in G1 and increase at least 4-fold as cells progress towards M phase. These data suggest that in human cells, as in fission yeast, the accumulation of CDC25 mitotic inducer during G2 may play a key role in regulating the timing of mitosis.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular / methods
  • Gene Expression
  • Gene Library
  • HeLa Cells / cytology
  • Humans
  • Interphase*
  • Kinetics
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotide Probes
  • Proteins / genetics*
  • RNA, Messenger / genetics
  • Schizosaccharomyces / genetics*
  • Sequence Homology, Nucleic Acid
  • cdc25 Phosphatases

Substances

  • Oligonucleotide Probes
  • Proteins
  • RNA, Messenger
  • cdc25 Phosphatases

Associated data

  • GENBANK/M34065