Abstract
Reactive oxygen species (ROS) caused oxidative stress plays a key role in carcinogenesis. The POU domain transcription factor Oct-1 and catalase is closely associated with ROS. However, a correlation between these two key proteins has not been demonstrated before. In this report, we show that Oct-1 acts as an activator of catalase, by binding to the catalase promoter in hepatocellular carcinoma (HCC) cell lines. In addition, we suggest that Oct-1 is downregulated by ROS via CpG island methylation in its promoter. These findings contribute to a better understanding of the epigenetic changes induced by ROS in the process of carcinogenesis.
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylcysteine / pharmacology
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Azacitidine / analogs & derivatives
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Azacitidine / pharmacology
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Carcinoma, Hepatocellular / enzymology
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Carcinoma, Hepatocellular / genetics
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Carcinoma, Hepatocellular / pathology
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Catalase / genetics*
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Catalase / metabolism
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Cell Line, Tumor
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CpG Islands / drug effects
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CpG Islands / genetics*
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DNA Methylation / drug effects*
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Decitabine
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Down-Regulation / drug effects*
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Enzyme Activation / drug effects
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Enzymologic / genetics
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Gene Expression Regulation, Neoplastic / drug effects
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Gene Expression Regulation, Neoplastic / genetics
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Humans
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Liver Neoplasms / enzymology
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Liver Neoplasms / genetics
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Liver Neoplasms / pathology
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Neoplasm Invasiveness
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Octamer Transcription Factor-1 / genetics*
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Promoter Regions, Genetic / drug effects
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Promoter Regions, Genetic / genetics*
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Reactive Oxygen Species / pharmacology*
Substances
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Octamer Transcription Factor-1
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Reactive Oxygen Species
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Decitabine
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Catalase
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Azacitidine
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Acetylcysteine