Silencing of miR-124 induces neuroblastoma SK-N-SH cell differentiation, cell cycle arrest and apoptosis through promoting AHR

Tsui-Chin Huang; Hsin-Yi Chang; Cheng-Yu Chen; Pei-Yi Wu; Hsinyu Lee; Yung-Feng Liao; Wen-Ming Hsu; Hsuan-Cheng Huang; Hsueh-Fen Juan

doi:10.1016/j.febslet.2011.10.025

Silencing of miR-124 induces neuroblastoma SK-N-SH cell differentiation, cell cycle arrest and apoptosis through promoting AHR

FEBS Lett. 2011 Nov 16;585(22):3582-6. doi: 10.1016/j.febslet.2011.10.025. Epub 2011 Oct 20.

Authors

Tsui-Chin Huang¹, Hsin-Yi Chang, Cheng-Yu Chen, Pei-Yi Wu, Hsinyu Lee, Yung-Feng Liao, Wen-Ming Hsu, Hsuan-Cheng Huang, Hsueh-Fen Juan

Affiliation

¹ Department of Life Science, National Taiwan University, Taipei, Taiwan.

PMID: 22024478
DOI: 10.1016/j.febslet.2011.10.025

Abstract

Neuroblastoma is the most common extracranial solid tumor in children. We investigate whether miR-124, the abundant neuronal miRNA, plays a pivotal role in neuroblastoma. Knockdown of miR-124 promotes neuroblastoma SK-N-SH cell differentiation, cell cycle arrest and apoptosis. Further miR-124 is predicted to target aryl hydrocarbon receptor (AHR) which may promote neuroblastoma cell differentiation. We validate that miR-124 may suppress the expression of AHR by targeting its 3'-UTR. These results suggest that miR-124 could serve as a potential therapeutic target of neuroblastoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Cell Cycle Checkpoints
Cell Cycle*
Cell Differentiation*
Gene Silencing*
Humans
MicroRNAs / genetics*
Neuroblastoma / genetics*
Neuroblastoma / metabolism
Neuroblastoma / pathology
Receptors, Aryl Hydrocarbon / genetics*
Receptors, Aryl Hydrocarbon / metabolism

Substances

MIRN124 microRNA, human
MicroRNAs
Receptors, Aryl Hydrocarbon