Induction of the Escherichia coli cysK gene by genetic and environmental factors

Kaneyoshi Yamamoto; Taku Oshima; Gen Nonaka; Hisao Ito; Akira Ishihama

doi:10.1111/j.1574-6968.2011.02364.x

Induction of the Escherichia coli cysK gene by genetic and environmental factors

FEMS Microbiol Lett. 2011 Oct;323(1):88-95. doi: 10.1111/j.1574-6968.2011.02364.x. Epub 2011 Aug 11.

Authors

Kaneyoshi Yamamoto¹, Taku Oshima, Gen Nonaka, Hisao Ito, Akira Ishihama

Affiliation

¹ Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Koganei, Tokyo, Japan. kanyamam@hosei.ac.jp

PMID: 22092684
DOI: 10.1111/j.1574-6968.2011.02364.x

Abstract

Cysteine synthase A encoded by cysK catalyzes the synthesis of cysteine from O-acetylserine. Expression of cysK in Escherichia coli is under the control of CysB, a LysR family transcription factor. Herein we showed that the expression of cysK is regulated by several genetic and environmental factors in addition to CysB: two genetic factors, OmpR and CysE, and lithium. Based on the findings, we constructed the high-level expression system of cysK.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / metabolism
Cysteine Synthase / biosynthesis*
Escherichia coli / enzymology*
Escherichia coli / genetics
Escherichia coli Proteins / biosynthesis*
Escherichia coli Proteins / metabolism
Gene Expression Regulation, Bacterial*
Gene Expression Regulation, Enzymologic*
Lithium / metabolism
Serine O-Acetyltransferase / metabolism
Trans-Activators / metabolism
Transcriptional Activation*

Substances

Bacterial Proteins
Escherichia coli Proteins
Trans-Activators
osmolarity response regulator proteins
Lithium
CysE protein, E coli
Serine O-Acetyltransferase
Cysteine Synthase