Polymorphic variations in manganese superoxide dismutase (MnSOD), glutathione peroxidase-1 (GPX1), and catalase (CAT) contribute to elevated plasma triglyceride levels in Chinese patients with type 2 diabetes or diabetic cardiovascular disease

Mol Cell Biochem. 2012 Apr;363(1-2):85-91. doi: 10.1007/s11010-011-1160-3. Epub 2011 Dec 14.

Abstract

Manganese superoxide dismutase (MnSOD), glutathione peroxidase-1 (GPX1), and catalase (CAT) provide the primary antioxidant defense system. Impaired antioxidant defense increases oxidative stress and contributes to the development of type 2 diabetes and diabetic cardiovascular disease (CVD). We preformed a case-control study in Chinese type 2 diabetes patients, to determine if the MnSOD Val16Ala (T→C), GPX1 Pro198Leu (C→T), and CAT -262C/T (C→T) functional polymorphisms contribute to the development of type 2 diabetes or diabetic CVD. Patients with type 2 diabetes (n = 168) were divided into the non-CVD group (n = 83, >10 year since diagnosis) and CVD group (n = 85, history of ischemic CVD). Genotyping was performed using PCR-restriction fragment length polymorphism (PCR-RFLP) or PCR-based direct sequencing. The genotypic distribution in the non-CVD- and CVD-group and the clinical parameters in genotypic groups were not significantly different in the three polymorphic sites, respectively. Among eight genotypic combinations, the most common TT+CC+CC genotype (59.5%) was associated with higher triglyceride levels than the TT+CT+CC genotype, the second frequent one (14.9%; 1.77 ± 0.12 vs. 1.21 ± 0.11 mmol/l, P = 0.001), and all non-TT+CC+CC genotypes (40.5%; 1.77 ± 0.12 vs. 1.43 ± 0.12 mmol/l, P = 0.048). In the CVD group, significantly elevated triglyceride levels were also observed in patients with TT+CC+CC compared to patients with TT+CT+CC (2.00 ± 0.18 vs. 1.37 ± 0.16 mmol/l, P = 0.018) or non-TT+CC+CC genotypes (2.00 ± 0.18 vs. 1.65 ± 0.19 mmol/l, P = 0.070). The common MnSOD, GPX1, and CAT TT+CC+CC genotype may contribute to hypertriglyceridemia in Chinese patients with type 2 diabetes or diabetic CVD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Asian People / genetics*
  • Cardiovascular Diseases / blood
  • Cardiovascular Diseases / enzymology
  • Cardiovascular Diseases / ethnology
  • Cardiovascular Diseases / genetics*
  • Case-Control Studies
  • Catalase / genetics*
  • Chi-Square Distribution
  • China / epidemiology
  • Diabetes Complications / blood
  • Diabetes Complications / enzymology
  • Diabetes Complications / ethnology
  • Diabetes Complications / genetics*
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / enzymology
  • Diabetes Mellitus, Type 2 / ethnology
  • Diabetes Mellitus, Type 2 / genetics*
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Glutathione Peroxidase / genetics*
  • Glutathione Peroxidase GPX1
  • Humans
  • Hypertriglyceridemia / blood
  • Hypertriglyceridemia / enzymology
  • Hypertriglyceridemia / ethnology
  • Hypertriglyceridemia / genetics*
  • Male
  • Phenotype
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Risk Assessment
  • Risk Factors
  • Superoxide Dismutase / genetics*
  • Triglycerides / blood*

Substances

  • Triglycerides
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • superoxide dismutase 2
  • Glutathione Peroxidase GPX1
  • GPX1 protein, human