Abstract
In this study, we identified five miRNAs highly expressed in the small-cell lung cancer (SCLC) cell line NCI-H209. Among them, the expression levels of miR-375 were dramatically elevated in all SCLC cell lines examined, coincident with the expression of the transcription factor achaete-scute complex homolog 1 (ASCL1). Moreover, miR-375 was upregulated and correlated with ASCL1 in the cell lines generated from mouse SCLC-like tumors as well. Dual-luciferase assays further showed that ASCL1 activated the expression of miR-375 by binding to the three E-box elements in the miR-375 promoter. These results imply a role of ASCL1 in SCLC via the upregulation of miR-375.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Basic Helix-Loop-Helix Transcription Factors / genetics
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Basic Helix-Loop-Helix Transcription Factors / metabolism*
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Binding Sites / genetics
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Carcinoma, Small Cell / genetics*
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Carcinoma, Small Cell / metabolism*
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Cell Line, Tumor
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DNA Primers / genetics
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E-Box Elements
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Humans
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Lung Neoplasms / genetics*
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Lung Neoplasms / metabolism*
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Mice
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MicroRNAs / genetics*
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MicroRNAs / metabolism*
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Promoter Regions, Genetic
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Transcriptional Activation
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Up-Regulation
Substances
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ASCL1 protein, human
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Ascl1 protein, mouse
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Basic Helix-Loop-Helix Transcription Factors
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DNA Primers
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MIRN375 microRNA, human
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MicroRNAs
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Mirn375 microRNA, mouse