Abstract
The presence of three different HBV genotypes (A, C and D) in Eastern India provided us a unique opportunity to study HBV pre-S mutants in these genotypes and subtypes among the same ethnic population. Furthermore, we also aimed to investigate the association of the HBV pre-S mutation with clinical outcome. Pre-S1-S2 and S gene was amplified and sequenced from 86 HBsAg positive study subjects with varying clinical manifestation. The genetic variability in the pre-S region (mutations) was studied with respect to different HBV genotypes, subtypes and different clinical categories. Six different types of HBV pre-S mutations were detected in 25 cases (29.07%), among which pre-S2 start codon mutation (28.0%) and pre-S2 deletion (24.0%) were most common. Pre-S mutation was highest in HBV/C (7/18; 38.89%) followed by HBV/A (9/27; 33.33%) and HBV/D (9/40; 22.50%). Pre-S1 deletion is common in HBV/D, whereas pre-S2 start codon mutation and pre-S2 deletions are frequent among HBV/A and HBV/C, respectively. Interestingly, in HBV/A and HBV/C the tendency of mutation/deletion increases from pre-S1 to pre-S2 region while in HBV/D the opposite tendency was observed. A significantly higher association of pre-S mutation (p=0.013) and pre-S2 deletion/ablation (p=0.016) was found among the HBeAg negative cases. Pre-S1 deletion and pre-S2 deletion were common among the ASC and CLD cases respectively, while pre-S2 start codon mutation was significantly associated with cirrhosis (p<0.05). The study underscores the association of types of pre-S mutations with particular HBV genotype and clinical outcome in the study population.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Child
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Female
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Genotype*
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Hepatitis B / epidemiology*
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Hepatitis B Surface Antigens / genetics*
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Hepatitis B e Antigens / immunology
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Hepatitis B virus / genetics*
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Hepatitis B virus / immunology
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Humans
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India / epidemiology
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Male
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Middle Aged
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Molecular Sequence Data
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Prevalence
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Prognosis
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RNA Splice Sites
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Recombination, Genetic
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Sequence Deletion*
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Young Adult
Substances
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Hepatitis B Surface Antigens
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Hepatitis B e Antigens
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RNA Splice Sites
Associated data
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GENBANK/DQ315776
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GENBANK/DQ315777
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GENBANK/DQ315778
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GENBANK/DQ315779
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GENBANK/DQ315780
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GENBANK/DQ315781
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GENBANK/DQ315782
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GENBANK/DQ315783
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GENBANK/DQ315784
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GENBANK/DQ315785
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GENBANK/DQ315786
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GENBANK/JN107815
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GENBANK/JN107816
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GENBANK/JN107817
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GENBANK/JN107818
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GENBANK/JN107819
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GENBANK/JN107820
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GENBANK/JN107821
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GENBANK/JN107822
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GENBANK/JN107823
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GENBANK/JN107824
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GENBANK/JN107825
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GENBANK/JN107826
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GENBANK/JN107827
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GENBANK/JN107828
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GENBANK/JN107829
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GENBANK/JN107830
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GENBANK/JN107831
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GENBANK/JN107832
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GENBANK/JN107833
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GENBANK/JN107834
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GENBANK/JN107835
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GENBANK/JN107836
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GENBANK/JN107837
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GENBANK/JN107838
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GENBANK/JN107839
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GENBANK/JN107840
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GENBANK/JN107841
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GENBANK/JN107842
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GENBANK/JN107843
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GENBANK/JN107844
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GENBANK/JN107845
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GENBANK/JN107846
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GENBANK/JN107847
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GENBANK/JN107848
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GENBANK/JN107849
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GENBANK/JN107850
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GENBANK/JN107851
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GENBANK/JN107852
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GENBANK/JN107853
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GENBANK/JN107854
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GENBANK/JN107855
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GENBANK/JN107856
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GENBANK/JN107857
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GENBANK/JN107858
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GENBANK/JN107859
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GENBANK/JN107860
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GENBANK/JN107861
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GENBANK/JN107862
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GENBANK/JN107863
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GENBANK/JN107864
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GENBANK/JN107865
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GENBANK/JN107866
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GENBANK/JN107867
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GENBANK/JN107868
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GENBANK/JN107869
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GENBANK/JN107870
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GENBANK/JN107871
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GENBANK/JN107872
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GENBANK/JN107873
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GENBANK/JN107874
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GENBANK/JN107875
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GENBANK/JN107876
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GENBANK/JN107877
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GENBANK/JN107878
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GENBANK/JN107879
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GENBANK/JN107880
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GENBANK/JN107881
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GENBANK/JN107882
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GENBANK/JN107883
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GENBANK/JN107884
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GENBANK/JN107885
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GENBANK/JN107886
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GENBANK/JN107887
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GENBANK/JN107888
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GENBANK/JN107889