Abstract
TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus
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Cell Nucleus / genetics
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Cell Nucleus / metabolism*
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Cell Nucleus / virology
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Cytoplasm / genetics
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Cytoplasm / metabolism
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Cytoplasm / virology
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HIV Infections / genetics
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HIV Infections / metabolism*
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HIV Infections / virology*
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HIV-1 / genetics
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HIV-1 / physiology*
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Humans
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Protein Binding
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Virus Integration*
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Virus Replication*
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beta Karyopherins / genetics
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beta Karyopherins / metabolism*
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gag Gene Products, Human Immunodeficiency Virus / genetics
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gag Gene Products, Human Immunodeficiency Virus / metabolism*
Substances
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TNPO3 protein, human
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beta Karyopherins
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gag Gene Products, Human Immunodeficiency Virus