Transcription expression and clinical significance of dishevelled-3 mRNA and δ-catenin mRNA in pleural effusions from patients with lung cancer

Clin Dev Immunol. 2012:2012:904946. doi: 10.1155/2012/904946. Epub 2012 Feb 23.

Abstract

Objective: To evaluate diagnostic utility of Dishevelled-3 (DVL-3) mRNA and δ-catenin mRNA expression in pleural effusions of patients with lung cancer.

Methods: DVL-3 mRNA and δ-catenin mRNA levels were assessed by performing RT-PCR on pleural effusion specimens from patients with lung cancer (n = 75) and with lung benign disease (n = 51).

Results: The expressions of DVL-3 mRNA and δ-catenin mRNA were significantly higher in malignant than in benign lung disease (P < 0.01) and were obviously higher than cytology in adenocarcinoma (P < 0.01). In single use, DVL-3 mRNA had the highest specificity (94.1%) and PPV (95.7%), whereas δ-catenin mRNA had the highest sensitivity (92.0%) and NPV (88.5%). When combinations of markers were evaluated together, DVL-3 mRNA and δ-catenin mRNA gave a high-diagnostic performance: sensitivity of 100.0%, NPV of 100.0%, and accuracy of 96.0%, respectively.

Conclusion: As molecular markers of detecting pleural micrometastasis, DVL-3 mRNA and δ-catenin mRNA are helpful to diagnose the cancer cells in pleural effusions of patients with lung cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics*
  • Adaptor Proteins, Signal Transducing / metabolism
  • Adenocarcinoma / diagnosis*
  • Adenocarcinoma / genetics
  • Adenocarcinoma / metabolism
  • Adenocarcinoma of Lung
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / metabolism
  • Case-Control Studies
  • Catenins / genetics*
  • Catenins / metabolism
  • Delta Catenin
  • Dishevelled Proteins
  • Female
  • Gene Expression
  • Humans
  • Lung Neoplasms / diagnosis*
  • Lung Neoplasms / genetics
  • Lung Neoplasms / metabolism
  • Male
  • Middle Aged
  • Neoplasm Micrometastasis / diagnosis*
  • Neoplasm Micrometastasis / genetics
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • Pleural Effusion / genetics
  • Pleural Effusion / metabolism
  • Pneumonia / diagnosis
  • Pneumonia / genetics
  • Pneumonia / metabolism
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics
  • Sensitivity and Specificity
  • Tuberculosis / diagnosis
  • Tuberculosis / genetics
  • Tuberculosis / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • Biomarkers, Tumor
  • Catenins
  • DVL3 protein, human
  • Dishevelled Proteins
  • Phosphoproteins
  • RNA, Messenger
  • Delta Catenin