Abstract
Objective:
To evaluate diagnostic utility of Dishevelled-3 (DVL-3) mRNA and δ-catenin mRNA expression in pleural effusions of patients with lung cancer.
Methods:
DVL-3 mRNA and δ-catenin mRNA levels were assessed by performing RT-PCR on pleural effusion specimens from patients with lung cancer (n = 75) and with lung benign disease (n = 51).
Results:
The expressions of DVL-3 mRNA and δ-catenin mRNA were significantly higher in malignant than in benign lung disease (P < 0.01) and were obviously higher than cytology in adenocarcinoma (P < 0.01). In single use, DVL-3 mRNA had the highest specificity (94.1%) and PPV (95.7%), whereas δ-catenin mRNA had the highest sensitivity (92.0%) and NPV (88.5%). When combinations of markers were evaluated together, DVL-3 mRNA and δ-catenin mRNA gave a high-diagnostic performance: sensitivity of 100.0%, NPV of 100.0%, and accuracy of 96.0%, respectively.
Conclusion:
As molecular markers of detecting pleural micrometastasis, DVL-3 mRNA and δ-catenin mRNA are helpful to diagnose the cancer cells in pleural effusions of patients with lung cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics*
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Adaptor Proteins, Signal Transducing / metabolism
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Adenocarcinoma / diagnosis*
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma of Lung
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Adult
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Aged
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Aged, 80 and over
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Biomarkers, Tumor / genetics*
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Biomarkers, Tumor / metabolism
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Case-Control Studies
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Catenins / genetics*
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Catenins / metabolism
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Delta Catenin
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Dishevelled Proteins
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Female
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Gene Expression
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Humans
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Lung Neoplasms / diagnosis*
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Male
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Middle Aged
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Neoplasm Micrometastasis / diagnosis*
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Neoplasm Micrometastasis / genetics
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Phosphoproteins / genetics*
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Phosphoproteins / metabolism
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Pleural Effusion / genetics
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Pleural Effusion / metabolism
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Pneumonia / diagnosis
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Pneumonia / genetics
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Pneumonia / metabolism
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RNA, Messenger / biosynthesis*
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RNA, Messenger / genetics
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Sensitivity and Specificity
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Tuberculosis / diagnosis
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Tuberculosis / genetics
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Tuberculosis / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Biomarkers, Tumor
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Catenins
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DVL3 protein, human
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Dishevelled Proteins
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Phosphoproteins
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RNA, Messenger
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Delta Catenin