Human macrophage scavenger receptors: primary structure, expression, and localization in atherosclerotic lesions

A Matsumoto; M Naito; H Itakura; S Ikemoto; H Asaoka; I Hayakawa; H Kanamori; H Aburatani; F Takaku; H Suzuki

doi:10.1073/pnas.87.23.9133

Human macrophage scavenger receptors: primary structure, expression, and localization in atherosclerotic lesions

Proc Natl Acad Sci U S A. 1990 Dec;87(23):9133-7. doi: 10.1073/pnas.87.23.9133.

Authors

A Matsumoto¹, M Naito, H Itakura, S Ikemoto, H Asaoka, I Hayakawa, H Kanamori, H Aburatani, F Takaku, H Suzuki, et al.

Affiliation

¹ Third Department of Internal Medicine, University of Tokyo, Japan.

Abstract

Two types of cDNAs for human macrophage scavenger receptors were cloned from a cDNA library derived from the phorbol ester-treated human monocytic cell line THP-1. The type I and type II human scavenger receptors encoded by these cDNAs are homologous (73% and 71% amino acid identity) to their previously characterized bovine counterparts and consist of six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), alpha-helical coiled-coil (IV), collagen-like (V), and a type-specific C-terminal (VI). The receptor gene is located on human chromosome 8. The human receptors expressed in CHO-K1 cells mediated endocytosis of modified low density lipoproteins. Two mRNAs, 4.0 and 3.2 kilobases, have been detected in human liver, placenta, and brain. Immunohistochemical studies using an anti-peptide antibody which recognizes human scavenger receptors indicated the presence of the scavenger receptors in the macrophages of lipid-rich atherosclerotic lesions, suggesting the involvement of scavenger receptors in atherogenesis.

Publication types

Comparative Study

MeSH terms

Amino Acid Sequence
Animals
Aorta / immunology
Aorta / pathology
Arteriosclerosis / immunology*
Arteriosclerosis / pathology
Base Sequence
Cattle
Cell Line
Chromosome Mapping
Chromosomes, Human, Pair 8*
Cloning, Molecular
DNA / genetics
Gene Expression / drug effects
Gene Library
Humans
Macrophages / immunology*
Membrane Proteins*
Molecular Sequence Data
Muscle, Smooth, Vascular / immunology
Muscle, Smooth, Vascular / pathology
Peptides / chemical synthesis
Polymerase Chain Reaction
Receptors, Immunologic / analysis
Receptors, Immunologic / genetics*
Receptors, Lipoprotein*
Receptors, Scavenger
Scavenger Receptors, Class A
Scavenger Receptors, Class B
Sequence Homology, Nucleic Acid
Tetradecanoylphorbol Acetate / pharmacology
Transfection

Substances

MSR1 protein, human
Membrane Proteins
Peptides
Receptors, Immunologic
Receptors, Lipoprotein
Receptors, Scavenger
Scarb1 protein, mouse
Scavenger Receptors, Class A
Scavenger Receptors, Class B
DNA
Tetradecanoylphorbol Acetate

Associated data

GENBANK/D90187
GENBANK/D90188