Abstract
Here we show that activation of the canonical WNT/β-catenin pathway increases the expression of stem cell genes and promotes the migratory and invasive capacity of glioblastoma. Modulation of WNT signaling alters the expression of epithelial-to-mesenchymal transition activators, suggesting a role of this process in the regulation of glioma motility. Using immunohistochemistry in patient-derived glioblastoma samples we showed higher numbers of cells with intranuclear signal for β-catenin in the infiltrating edge of tumor compared to central tumor parenchyma. These findings suggest that canonical WNT/β-catenin pathway is a critical regulator of GBM invasion and may represent a potential therapeutic target.
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AC133 Antigen
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Antigens, CD / genetics
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Antigens, CD / metabolism
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Cell Differentiation / genetics
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Cell Growth Processes / genetics
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Cell Line, Tumor
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Cell Movement / genetics
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Cell Movement / physiology*
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Epithelial-Mesenchymal Transition*
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Glioblastoma / genetics
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Glioblastoma / metabolism*
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Glioblastoma / pathology*
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Glycoproteins / genetics
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Glycoproteins / metabolism
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HEK293 Cells
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism*
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Humans
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Immunohistochemistry
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism
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Neoplasm Invasiveness
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Neoplastic Stem Cells / metabolism
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Neoplastic Stem Cells / pathology
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Peptides / genetics
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Peptides / metabolism
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Signal Transduction
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Wnt Proteins / genetics
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Wnt Proteins / metabolism
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Wnt Signaling Pathway*
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Zinc Finger E-box-Binding Homeobox 1
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beta Catenin / genetics
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beta Catenin / metabolism
Substances
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AC133 Antigen
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Antigens, CD
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DKK1 protein, human
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Glycoproteins
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Homeodomain Proteins
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Intercellular Signaling Peptides and Proteins
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Peptides
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Transcription Factors
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Wnt Proteins
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ZEB1 protein, human
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Zinc Finger E-box-Binding Homeobox 1
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beta Catenin