Abstract
Background:
This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC).
Methods:
Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets.
Results:
Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.
Conclusions:
These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.
MeSH terms
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Adaptor Proteins, Signal Transducing / biosynthesis
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Adaptor Proteins, Signal Transducing / genetics*
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Adaptor Proteins, Signal Transducing / metabolism
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Breast Neoplasms / genetics*
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology
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Cell Growth Processes / genetics
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Cell Line, Tumor
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Cell Movement / genetics*
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Cytoskeletal Proteins / biosynthesis
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Cytoskeletal Proteins / genetics*
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Cytoskeletal Proteins / metabolism
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Down-Regulation
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Inhibitor of Apoptosis Proteins / biosynthesis
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Inhibitor of Apoptosis Proteins / genetics*
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Inhibitor of Apoptosis Proteins / metabolism
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LIM Domain Proteins / biosynthesis
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LIM Domain Proteins / genetics*
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LIM Domain Proteins / metabolism
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MicroRNAs / biosynthesis
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Small Interfering / administration & dosage
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RNA, Small Interfering / genetics
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Receptor, ErbB-2 / metabolism
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Receptors, Estrogen / metabolism
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Receptors, Progesterone / metabolism
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Survivin
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Transfection
Substances
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Adaptor Proteins, Signal Transducing
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BIRC5 protein, human
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Cytoskeletal Proteins
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Inhibitor of Apoptosis Proteins
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LASP1 protein, human
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LIM Domain Proteins
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MIRN203 microRNA, human
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MicroRNAs
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RNA, Messenger
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RNA, Small Interfering
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Receptors, Estrogen
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Receptors, Progesterone
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Survivin
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Receptor, ErbB-2