Abstract
Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterization shows that the recombinant protein is identical with the natural protein and is devoid of posttranslational glycosylation, sulfation, and phosphorylation. The two C-terminal amino acids are proteolytically removed from the mature recombinant MGSA, indicating a length of 71 instead of the predicted 73 amino acids. The recombinant MGSA is mitogenically active on the Hs294T melanoma cells. The purified MGSA competes with interleukin 8 for binding to neutrophil receptors and exhibits neutrophil chemotactic activity equivalent to that of interleukin 8.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Binding, Competitive
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Cell Division / drug effects
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Cell Line
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Chemokine CXCL1
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Chemokines, CXC*
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Chemotaxis, Leukocyte / drug effects
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Cricetinae
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Cricetulus
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DNA / genetics
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Fibroblasts / metabolism
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Genetic Vectors
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Growth Substances* / genetics
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Growth Substances* / isolation & purification
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Growth Substances* / pharmacology*
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Humans
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Intercellular Signaling Peptides and Proteins*
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Interleukin-8 / metabolism
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Melanoma / pathology
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Molecular Sequence Data
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Neoplasm Proteins / genetics
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Neoplasm Proteins / isolation & purification
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Neoplasm Proteins / pharmacology
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Neutrophils / drug effects
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Neutrophils / metabolism
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / pharmacology
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Tumor Cells, Cultured / drug effects
Substances
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CXCL1 protein, human
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Chemokine CXCL1
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Chemokines, CXC
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Interleukin-8
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Neoplasm Proteins
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Recombinant Fusion Proteins
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DNA