Abstract
Using a series of detailed experiments, Zhang and colleagues establish that the prostate cancer RNA chimera SLC45A3-ELK4 is generated by cis-splicing between the 2 adjacent genes and does not involve DNA rearrangements or trans-splicing. The chimera expression is induced by androgen treatment likely by overcoming the read-through block imposed by the intergenic CCCTC insulators bound by CCCTC-binding factor repressor protein. The chimeric transcript, but not wild-type ELK4, is shown to augment prostate cancer cell proliferation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Comment
MeSH terms
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Animals
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Cell Proliferation*
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Humans
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Male
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Membrane Transport Proteins / genetics*
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Monosaccharide Transport Proteins
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Prostatic Neoplasms / genetics*
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RNA Splicing*
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Recombinant Fusion Proteins / genetics*
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ets-Domain Protein Elk-4 / genetics*
Substances
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ELK4 protein, human
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Membrane Transport Proteins
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Monosaccharide Transport Proteins
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Recombinant Fusion Proteins
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SLC45a3 protein, human
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ets-Domain Protein Elk-4