Arvelexin inhibits colonic inflammation by suppression of NF-κB activation in dextran sulfate sodium-induced mice and TNF-α-induced colonic epithelial cells

J Agric Food Chem. 2012 Aug 1;60(30):7398-407. doi: 10.1021/jf3009553. Epub 2012 Jul 23.

Abstract

Recently, we reported the anti-inflammatory effects of arvelexin isolated from Brassica rapa in macrophages. In the present study, the effects of arvelexin were investigated in a dextran sulfate sodium (DSS)-induced colitis mouse model and in a cellular model. In the DSS-induced colitis model, arvelexin significantly reduced the severity of colitis, as assessed by disease activity, colonic damage, neutrophil infiltration, and levels of colonic iNOS. Moreover, arvelexin inhibited the expressions of IL-8, IP-10, ICAM-1, and VCAM-1 in HT-29 colonic epithelial cells. Arvelexin also inhibited the TNF-α-induced adhesion of U937 monocytic cells to HT-29 cells. Furthermore, arvelexin reduced p65 NF-κB subunit translocation to the nucleus and IκBα degradation in the colonic tissues and in TNF-α-induced HT-29 cells. These results demonstrate that the ameliorative effects of arvelexin on colonic injury are mainly related to its ability to inhibit the inflammatory responses via NF-κB inactivation, and support its possible therapeutic role in colitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brassica rapa / chemistry
  • Colitis / chemically induced
  • Colitis / drug therapy
  • Colon / cytology
  • Colon / drug effects*
  • Colon / physiopathology
  • Dextran Sulfate
  • Disease Models, Animal
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • HT29 Cells
  • Humans
  • I-kappa B Proteins / genetics
  • I-kappa B Proteins / metabolism
  • Indoles / pharmacology*
  • Inflammation / drug therapy*
  • Intercellular Adhesion Molecule-1 / genetics
  • Intercellular Adhesion Molecule-1 / metabolism
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism
  • Male
  • Mice
  • Mice, Inbred ICR
  • NF-KappaB Inhibitor alpha
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / genetics*
  • NF-kappa B / metabolism
  • Neutrophil Infiltration / drug effects
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / metabolism
  • Plant Roots / chemistry
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism*
  • U937 Cells
  • Vascular Cell Adhesion Molecule-1 / genetics
  • Vascular Cell Adhesion Molecule-1 / metabolism

Substances

  • I-kappa B Proteins
  • Indoles
  • Interleukin-8
  • NF-kappa B
  • NFKBIA protein, human
  • Nfkbia protein, mouse
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1
  • arvelexin
  • Intercellular Adhesion Molecule-1
  • NF-KappaB Inhibitor alpha
  • Dextran Sulfate
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse