Comparison of BD GeneOhm Cdiff and Seegene Seeplex ACE PCR assays using toxigenic Clostridium difficile culture for direct detection of tcdB from stool specimens

J Clin Microbiol. 2012 Nov;50(11):3765-7. doi: 10.1128/JCM.01440-12. Epub 2012 Sep 5.

Abstract

We evaluated the performances of 2 PCR assays (BD GeneOhm and Seegene ACE) for direct detection of tcdB from stool specimens. The concordance rate between BD and Seegene was 96.3%. The sensitivities, specificities, positive predictive values (PPVs), and negative predictive values (NPVs) of BD and Seegene were 95.7%, 96.5%, 91.8%, and 98.2% and 90.0%, 97.1%, 92.6%, and 96.0%, respectively.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Toxins / genetics*
  • Bacteriological Techniques / methods
  • Clostridioides difficile / genetics*
  • Clostridium Infections / diagnosis*
  • Feces / microbiology*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Polymerase Chain Reaction / methods*
  • Predictive Value of Tests
  • Sensitivity and Specificity

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • toxB protein, Clostridium difficile