Abstract
Selenium and exendin-4 exert antidiabetic effects by unknown mechanisms. Herein, we investigated their effects on the expression of glucagon-like peptide-1 receptor (GLP-1R), insulin receptor substrate-1 (IRS-1), and Raf-1 in the livers of rats with streptozotocin-induced diabetes. Diabetic rats were injected intraperitoneally with exendin-4 (0.03 μg/kg body weight) twice daily or treated with 5 ppm selenium as sodium selenite in drinking water for 4 weeks. Both selenium and exendin-4 reduced the hyperglycemia in diabetic rats. Induction of diabetes mellitus resulted in decreased level of GLP-1R and increased levels of IRS-1 and Raf-1 in the liver. Treatment of diabetic rats with selenium or exendin-4 resulted in increased level of GLP-1R and decreased levels of IRS-1 and Raf-1 in the liver, compared with the levels in diabetic rats. Therefore, the antidiabetic actions of selenium and exendin-4 involve their effects on GLP-1R, IRS-1, and Raf-1 levels in the liver.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blood Glucose
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Blotting, Western
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Diabetes Mellitus, Experimental / drug therapy
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Diabetes Mellitus, Experimental / metabolism
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Diabetes Mellitus, Experimental / pathology
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Exenatide
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Glucagon-Like Peptide-1 Receptor
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Hypoglycemic Agents / pharmacology
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Insulin / metabolism
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Insulin / pharmacology
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Insulin Receptor Substrate Proteins / genetics
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Insulin Receptor Substrate Proteins / metabolism*
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Liver / drug effects*
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Liver / metabolism
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Liver / pathology
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MAP Kinase Kinase Kinases / genetics
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MAP Kinase Kinase Kinases / metabolism*
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Male
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Peptides / administration & dosage
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Peptides / pharmacology*
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Proto-Oncogene Proteins c-raf
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Receptors, Glucagon / genetics
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Receptors, Glucagon / metabolism*
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Selenium / metabolism
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Selenium / pharmacology
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Sodium Selenite / administration & dosage
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Sodium Selenite / pharmacology*
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Streptozocin / administration & dosage
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Streptozocin / adverse effects
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Time Factors
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Venoms / administration & dosage
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Venoms / pharmacology*
Substances
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Blood Glucose
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Glp1r protein, rat
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Glucagon-Like Peptide-1 Receptor
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Hypoglycemic Agents
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Insulin
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Insulin Receptor Substrate Proteins
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Irs1 protein, rat
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Peptides
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RNA, Messenger
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Receptors, Glucagon
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Venoms
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Streptozocin
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Exenatide
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Proto-Oncogene Proteins c-raf
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Raf1 protein, rat
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MAP Kinase Kinase Kinases
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Selenium
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Sodium Selenite