Identification of a Rho family specific guanine nucleotide exchange factor, FLJ00018, as a novel actin-binding protein

Cell Signal. 2013 Jan;25(1):41-9. doi: 10.1016/j.cellsig.2012.09.015. Epub 2012 Sep 19.

Abstract

FLJ00018/PLEKHG2 is a guanine nucleotide exchange factor for the Rho family small GTPases. FLJ00018 is directly activated by heterotrimeric G protein Gβγ subunits. Using two-hybrid screening, we have identified non-muscle cytosolic actin as a binding partner of FLJ00018. We found that there were two actin-binding regions in FLJ00018 at the N-terminal region (150-283 amino acids) and at the C-terminal region (465-1386 amino acids). The overexpression of non-muscle cytosolic actin attenuated the FLJ00018-induced serum response element-dependent gene transcription. These results suggest that non-muscle cytosolic actin may be a negative regulator of FLJ00018 through its interaction with the Dbl homology domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Actins / metabolism*
  • Animals
  • GTP-Binding Protein beta Subunits / genetics
  • GTP-Binding Protein beta Subunits / metabolism
  • GTP-Binding Protein gamma Subunits / genetics
  • GTP-Binding Protein gamma Subunits / metabolism
  • Guanine Nucleotide Exchange Factors / chemistry
  • Guanine Nucleotide Exchange Factors / genetics
  • Guanine Nucleotide Exchange Factors / metabolism*
  • HEK293 Cells
  • Humans
  • Mice
  • NIH 3T3 Cells
  • Protein Binding
  • Protein Structure, Tertiary
  • Serum Response Element
  • Transcription, Genetic
  • Transfection
  • Two-Hybrid System Techniques

Substances

  • Actins
  • GTP-Binding Protein beta Subunits
  • GTP-Binding Protein gamma Subunits
  • Guanine Nucleotide Exchange Factors
  • PLEKHG2 protein, human