Association study of gene LPP in women with polycystic ovary syndrome

PLoS One. 2012;7(10):e46370. doi: 10.1371/journal.pone.0046370. Epub 2012 Oct 3.

Abstract

Background: Previous genome-wide association study (GWAS) of polycystic ovary syndrome (PCOS) in Han Chinese population has found that SNPs in LPP gene were nominally significant in PCOS patients (P around 10E-05). Replication of the GWAS was applied to further confirm the relationship between LPP gene and PCOS.

Methods: Three polymorphisms of LPP gene (rs715790, rs4449306, rs6782041) were selected and replicated in additional 1132 PCOS cases and 1142 controls. Genotyping of LPP gene was carried out by Taqman-MGB method.

Results: In rs715790, the allele frequency is significantly different between the PCOS group and the control group. Meta-analysis showed that the allele frequencies of the three SNPs rs715790 (P(meta) =1.89E-05, OR=1.23), rs4449306 (P(meta) =3.0E-04, OR=1.10), rs6782041 (P(meta) = 2.0E-04, OR=1.09), were significantly different between PCOS cases and controls.

Conclusions: Our results suggest that LPP gene might be a novel candidate for PCOS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytoskeletal Proteins / genetics*
  • Female
  • Genome-Wide Association Study
  • Humans
  • LIM Domain Proteins / genetics*
  • Polycystic Ovary Syndrome / genetics*
  • Polymorphism, Single Nucleotide
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Cytoskeletal Proteins
  • LIM Domain Proteins
  • LPP protein, human

Grants and funding

This research was supported by the Science research foundation item of no-earnings health vocation (201002013) and National Key Technology Research and Development Program (2011BAI17B00), National Basic Research Program of China (973 program) (2012CB944700, 2011CB944502), National Natural Science Foundation of China (30973170, 81000238). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.