Abstract
CD27(+) memory B cells are reduced in the blood of patients with chronic granulomatous disease (CGD) for reasons and consequences that remain unclear. Here we confirm not only decreased CD27(+) but also IgG(+) B cells in the blood of CGD patients compared with healthy donors (HDs). However, among IgG(+) B cells, the ratio of CD27(-) to CD27(+) was significantly higher in CGD patients compared with HDs. Similar to conventional memory B cells, CD27(-)IgG(+) B cells of CGD patients expressed activation markers and had undergone somatic hypermutation, albeit at levels lower than their CD27(+) counterparts. Functional analyses revealed slight reductions in frequencies of total IgG but not influenza-specific memory B-cell responses, as measured by Elispot in CGD patients compared with HDs. Serum IgG levels and influenza-specific antibodies were also normal in these CGD patients. Finally, we provide evidence that influenza-specific memory B cells can be present within the CD27(-)IgG(+) B-cell compartment. Together, these findings show that, despite reduced circulating CD27(+) memory B cells, CGD patients maintain an intact humoral immunologic memory, with potential contribution from CD27(-) B cells.
Publication types
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Research Support, N.I.H., Intramural
MeSH terms
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Adult
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B-Lymphocytes / immunology*
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B-Lymphocytes / metabolism
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Enzyme-Linked Immunospot Assay
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Female
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Flow Cytometry
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Granulomatous Disease, Chronic / blood
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Granulomatous Disease, Chronic / genetics
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Granulomatous Disease, Chronic / immunology*
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Humans
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Immunoglobulin G / blood
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Immunoglobulin G / immunology*
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Heavy Chains / immunology
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Immunoglobulin Variable Region / genetics
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Immunoglobulin Variable Region / immunology
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Immunoglobulin kappa-Chains / genetics
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Immunoglobulin kappa-Chains / immunology
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Immunoglobulin lambda-Chains / genetics
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Immunoglobulin lambda-Chains / immunology
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Immunologic Memory / genetics
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Immunologic Memory / immunology*
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Influenza A Virus, H1N1 Subtype / immunology
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Male
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology
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Mutation
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NADPH Oxidase 2
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NADPH Oxidases / genetics
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NADPH Oxidases / immunology
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Somatic Hypermutation, Immunoglobulin
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / blood
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / immunology*
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Young Adult
Substances
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Immunoglobulin G
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Immunoglobulin Heavy Chains
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Immunoglobulin Variable Region
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Immunoglobulin kappa-Chains
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Immunoglobulin lambda-Chains
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Membrane Glycoproteins
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Tumor Necrosis Factor Receptor Superfamily, Member 7
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CYBB protein, human
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NADPH Oxidase 2
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NADPH Oxidases
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neutrophil cytosolic factor 1