Abstract
Synaptojanin 2 (SYNJ2) is a phosphatidylinositol (PI) phosphatase that controls two distinct functions, clathrin-mediated endocytosis and tumor cell invadopodia formation and invasion. Here, we identify a number of novel SYNJ2 binding partners, several of which have previously been shown to be necessary for invadopodia formation or clathrin-mediated endocytosis. We focus on Src family kinases. We found that Src phosphorylates SYNJ2 on Tyr ( 490) , thereby stimulating SYNJ2 5'-phosphatase activity in vitro. We also provide evidence that Src-mediated phosphorylation of SYNJ2 contributes to invadopodia formation.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Blotting, Western
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Cell Membrane / metabolism
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Clathrin-Coated Vesicles / metabolism*
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Cortactin / genetics
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Cortactin / metabolism*
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Endocytosis
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Enzyme Activation
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HEK293 Cells
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Humans
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Immunoprecipitation
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Phosphoric Monoester Hydrolases / genetics
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Phosphoric Monoester Hydrolases / metabolism*
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Phosphorylation
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Plasmids / genetics
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Plasmids / metabolism
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Protein Binding
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Protein Interaction Mapping
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Proto-Oncogene Proteins c-fyn / genetics
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Proto-Oncogene Proteins c-fyn / metabolism*
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Transfection
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Tyrosine / metabolism
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src Homology Domains
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src-Family Kinases / genetics
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src-Family Kinases / metabolism
Substances
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CTTN protein, human
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Cortactin
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RNA, Small Interfering
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Recombinant Proteins
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Tyrosine
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FYN protein, human
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Proto-Oncogene Proteins c-fyn
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lyn protein-tyrosine kinase
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src-Family Kinases
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Phosphoric Monoester Hydrolases
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phosphoinositide 5-phosphatase