A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

Masayuki Takahashi; Reyad A Elbarbary; Aiko Nakashima; Mayumi Abe; Norihiro Watanabe; Miwako Narita; Masuhiro Takahashi; Masato Tamura; Tetsuo Yoshida; Masayuki Nashimoto

doi:10.1016/j.canlet.2012.10.016

A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

Cancer Lett. 2013 Jan 28;328(2):362-8. doi: 10.1016/j.canlet.2012.10.016. Epub 2012 Oct 22.

Authors

Masayuki Takahashi¹, Reyad A Elbarbary, Aiko Nakashima, Mayumi Abe, Norihiro Watanabe, Miwako Narita, Masuhiro Takahashi, Masato Tamura, Tetsuo Yoshida, Masayuki Nashimoto

Affiliation

¹ Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan.

PMID: 23092557
DOI: 10.1016/j.canlet.2012.10.016

Abstract

tRNase Z(L)-utilizing efficacious gene silencing is a gene control technology, which is based on the property that tRNase Z(L) can cleave any target RNA under the direction of an appropriate small guide RNA (sgRNA). To find therapeutic sgRNAs to cure hematological malignancies, we investigated behavior of heptamer-type sgRNA. We demonstrated that a heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA, can be taken up by cells without any transfection reagents and that it can induce apoptosis of the leukemia cells. Mouse xenograft experiments showed that a median survival of the mh1(Bcl-2)-treated mice was longer than that of the control mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis / genetics*
Cell Line, Tumor
Cell Proliferation
Female
Gene Silencing*
Genes, bcl-2*
HL-60 Cells
Humans
Leukemia / genetics*
Leukemia / metabolism
Mice
Mice, Nude
RNA, Messenger / metabolism
RNA, Small Untranslated
Transplantation, Heterologous

Substances

RNA, Messenger
RNA, Small Untranslated