Expression of promyelocytic leukemia protein and vascular endothelial growth factor in aqueous humor and vitreous fluid in patients with proliferative diabetic retinopathy

Takashi Katome; Kazuhiko Namekata; Takeshi Naito; Kentaro Semba; Xiaoli Guo; Chikako Harada; Takayuki Harada; Yoshinori Mitamura

doi:10.1016/j.diabres.2012.09.020

Expression of promyelocytic leukemia protein and vascular endothelial growth factor in aqueous humor and vitreous fluid in patients with proliferative diabetic retinopathy

Diabetes Res Clin Pract. 2012 Nov;98(2):e9-e11. doi: 10.1016/j.diabres.2012.09.020. Epub 2012 Oct 23.

Authors

Takashi Katome¹, Kazuhiko Namekata, Takeshi Naito, Kentaro Semba, Xiaoli Guo, Chikako Harada, Takayuki Harada, Yoshinori Mitamura

Affiliation

¹ Department of Ophthalmology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.

PMID: 23092969
DOI: 10.1016/j.diabres.2012.09.020

Abstract

Aims: To examine the expression of promyelocytic leukemia protein (PML) in the eye of proliferative diabetic retinopathy (PDR) patients.

Methods: PML mRNA levels were measured in proliferative membranes from 12 PDR patients and idiopathic epiretinal membranes from 5 control patients by quantitative RT-PCR. Protein levels of PML and vascular endothelial growth factor (VEGF) in aqueous humor and vitreous fluid samples from 34 PDR patients and 38 control patients were determined using ELISA.

Results: The PML mRNA expression levels in membrane samples, and the PML protein concentrations in aqueous humor and vitreous fluid samples were significantly lower in PDR patients than control patients. We observed a statistically significant inverse correlation between the concentrations of PML and VEGF in the aqueous humor and vitreous fluid of PDR patients.

Conclusion: PML may be a good candidate as a diagnostic marker and a therapeutic agent for PDR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aqueous Humor / metabolism*
Diabetic Retinopathy / genetics
Diabetic Retinopathy / metabolism*
Female
Humans
Male
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Promyelocytic Leukemia Protein
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors / genetics
Transcription Factors / metabolism*
Tumor Suppressor Proteins / genetics
Tumor Suppressor Proteins / metabolism*
Vascular Endothelial Growth Factor A / genetics
Vascular Endothelial Growth Factor A / metabolism*
Vitreous Body / metabolism*

Substances

Nuclear Proteins
Promyelocytic Leukemia Protein
RNA, Messenger
Transcription Factors
Tumor Suppressor Proteins
Vascular Endothelial Growth Factor A
PML protein, human