Ubiquitin C-terminal Hydrolase 37, a novel predictor for hepatocellular carcinoma recurrence, promotes cell migration and invasion via interacting and deubiquitinating PRP19

Ying Fang; Da Fu; Wenqing Tang; Yu Cai; Duan Ma; Huijun Wang; Ruyi Xue; Taotao Liu; Xiaowu Huang; Ling Dong; Hao Wu; Xizhong Shen

doi:10.1016/j.bbamcr.2012.11.020

Ubiquitin C-terminal Hydrolase 37, a novel predictor for hepatocellular carcinoma recurrence, promotes cell migration and invasion via interacting and deubiquitinating PRP19

Biochim Biophys Acta. 2013 Mar;1833(3):559-72. doi: 10.1016/j.bbamcr.2012.11.020. Epub 2012 Dec 4.

Authors

Ying Fang¹, Da Fu, Wenqing Tang, Yu Cai, Duan Ma, Huijun Wang, Ruyi Xue, Taotao Liu, Xiaowu Huang, Ling Dong, Hao Wu, Xizhong Shen

Affiliation

¹ The Department of Gastroenterology of Zhongshan Hospital, Fudan University, Shanghai, 200032, PR China.

PMID: 23220010
DOI: 10.1016/j.bbamcr.2012.11.020

Abstract

Ubiquitin C-terminal hydrolase 37 (UCH37) plays a crucial role in numerous biological processes and is also involved in oncogenesis. In this study, clinicopathologic data showed that UCH37 was over-expressed in hepatocellular carcinoma (HCC) cancerous tissues and was a significant predictor for time to recurrence (TTR). In vitro, we discovered that UCH37 could promote cell migration and invasion. Subsequently, we utilized Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to identify differentially expressed proteins in UCH37 over-expressing cells compared with the control cells, and found that PRP19, an essential RNA splicing factor, was up-regulated. The relationship between UCH37, PRP19 and the capability of cell migration and invasion was further confirmed. Collectively, this study demonstrated that UCH37 could promote cell migration and invasion in HCC cell lines through interacting and deubiquitinating PRP19, and suggested that UCH37 could be a novel predictor for HCC recurrence after curative resection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis
Blotting, Western
Carboxypeptidases / genetics
Carboxypeptidases / metabolism*
Carcinoma, Hepatocellular / metabolism*
Carcinoma, Hepatocellular / mortality
Carcinoma, Hepatocellular / secondary
Cell Adhesion
Cell Cycle
Cell Movement*
Cell Proliferation
DNA Repair Enzymes / antagonists & inhibitors
DNA Repair Enzymes / genetics
DNA Repair Enzymes / metabolism*
Female
Flow Cytometry
Fluorescent Antibody Technique
Humans
Immunoenzyme Techniques
Immunoprecipitation
Liver Neoplasms / metabolism*
Liver Neoplasms / mortality
Liver Neoplasms / pathology
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Recurrence, Local / metabolism*
Neoplasm Recurrence, Local / mortality
Neoplasm Recurrence, Local / pathology
Nuclear Proteins / antagonists & inhibitors
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Prognosis
Protein Processing, Post-Translational
RNA Splicing Factors
RNA, Messenger / genetics
RNA, Small Interfering / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Survival Rate
Ubiquitin / metabolism*
Ubiquitin Thiolesterase
Wound Healing

Substances

Nuclear Proteins
RNA Splicing Factors
RNA, Messenger
RNA, Small Interfering
Ubiquitin
Carboxypeptidases
UCHL5 protein, human
Ubiquitin Thiolesterase
DNA Repair Enzymes
PRPF19 protein, human